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KMID : 0363219960340040591
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Korean Journal of Dermatology
1996 Volume.34 No. 4 p.591 ~ p.599
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Detection of Type VIII Collagen mRNA in Scleroderma Fibroblasts
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Abstract
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Background :
@EN Type VII collagen is a relatively low abundance extracellular matrix protein among the collagenous molecules. Among the minor collagen, type VII collagen has been demon strated by a immunolocalization studies to be component of anchoring
fibrils and
structures extending perpendicularly from the lamina densa to the upper papillary dermis.
@ES Objective :
@EN The purpose of this study is to determine the expression of the type VII collagen gene in a group of scleroderma patients as compared to normal skin.
@ES Methods :
@EN We have examined the levels of type VII collagen mRNA using quantitative reverse transcription PCR and in situ hybridization in scleroderma skin fibroblasts. Immunofluorescent staining with ant-type VII collagen antibody was performed in
vitro
and
in vivo to evaluate the expression of type VII collagen at protein level.
@ES Results :
@EN 1. The ratio of type VII collagen/GAPDH RT-PCR product were 63.3±15.3 in scleroderma and 21.7±7.6 in normal fibroblasts by RT-PCR.
2. The expression of type VII collagen mRNA was considerably lower than type I in scleroder ma. A few positive signals by in situ hybridization with type VII collagen cDNA were shown in the dermis.
3. The staining was markedly enhanced in scleroderma fibroblasts and tissues compaired with ant-Type VII collagen antibody.
@ES Conclusion :
@EN RT-PCR and immunofluorescent staining with antibodies to type VII collagen shows enhanced gene expression in scleroderma skin fibroblasts. These data suggest that type VII collagen may be the main source of the sclerotic change of skin in
scleroderma.
(Kor J Dermatol 1996;34(4) : 591~99)
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KEYWORD
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